1. Sponsor Organization: NIH/NIEHS

2. Project Title: 06273-05 MOLECULAR MECHANISMS OF DIOXIN ACTION

3. Project Focus:

• Project Primary Focus: Human Health Effects
• Project Secondary Focus:

4. Description:

The long-term objective of this research proposal is to elucidate the biological responses to tetrachlorodibenzo-p-dioxin (TCDD; dioxin). Specifically, this proposal address the hypothesis that TCDD modifies gene expression patterns by interfering with the normal control mechanisms that regulate the steady state levels of tissue-specific transcription factors. The proposed research is based on the observation from our laboratory that TCDD induces expression of a transcription factor, AP-1, that has an essential role in differentiation, cell proliferation, and tumor promotion. The goal of the proposed experiments is to study the effect of TCDD exposure on theoxidative stress pathways operative on the expression of AP-1 and to analyze the expression of genes controlled by the factor. Major objectives of this work are, (1) to determine the mechanisms by which TCDD induces AP-1 and, (2) to ascertain whether the biological effects of TCDD are dependent on AP-1 induction.

5. References:

6. Inventory Category:

• Primary: Models
• Secondary:

7. Inventory Subcategory:

• Primary: Basic Research
• Secondary:

8. Keywords for Experimental System/Species:

• Species:
• Study Type:
  • Laboratory Study
• Fate and Transport:

9. Keywords for Experimental Endpoints:

• Health Effect:
• Hormonal Measures:
  • Stress Response, Ah Receptor,
• Level Of Study:
  • Molecular
  • Gene Expression
• Chemistry Metabolism:
• Life Stage:
• Risk Assessment:

10. Chemical Agents:

• Dioxins

11. Performing Institution:

• UNIVERSITY OF CINCINNATI

12. Contact:

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